Primary Identifier | MGI:3772666 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Kcnq1ot1 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A polyadenylation signal in the correct orientation followed by a floxed neo cassette was inserted downstream of the endogenous promoter. The neo cassette was removed by cre-mediated recombination. The expression of RNA upstream of the insertion and the absence of expression of RNA downstream of the insertion was confirmed by RNase protection assay and RT-PCR. |