| Primary Identifier | MGI:3771723 | Allele Type | Targeted |
| Gene | Rpe65 | Transmission | Germline |
| Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Two point mutations were introduced into exon 4 at codon 91 (CGA to TGG) to cause an arginine to tryptophan missense mutation (R91W). A floxed neomycin resistance cassette was also introduced into intron 6 for targeting purposes. Founder mice were mated with deleter mice to remove the neomycin cassette, leaving behind a single loxP site. Levels of the mutant protein are reduced by 95% compared to wild-type controls as determined by western blots of retinal extracts. |
