| Primary Identifier | MGI:3773921 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Itgax-SV40T,-EGFP)2Hao |
| Strain of Origin | (C57BL/6 x DBA/2)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Two lines were created with the same transgene vector (Mushi1 and Mushi2). Mushi1 expresses high levels of the transgene while Mushi2 expresses low levels. |
| molecularNote | The Itgax promoter drives dendritic cell-specific expression of a floxed cassette that contains the SV40 T-antigen gene with an inserted initiation site, an inserted perfect Kozak sequence and removal of the 5' and 3' untranslated regions followed by an IRES-EGFP reporter. The intron and polyadenylation signal of rabbit beta1 globin with a 3' frt site is located between the promoter and floxed cassette. Production of multiple transgene splice products was confirmed by RT-PCR on spleen extracts. |
