| First Author | Ishikawa K | Year | 2005 |
| Journal | Biochem Biophys Res Commun | Volume | 333 |
| Issue | 2 | Pages | 590-5 |
| PubMed ID | 15953585 | Mgi Jnum | J:132407 |
| Mgi Id | MGI:3775893 | Doi | 10.1016/j.bbrc.2005.05.155 |
| Citation | Ishikawa K, et al. (2005) Application of ES cells for generation of respiration-deficient mice carrying mtDNA with a large-scale deletion. Biochem Biophys Res Commun 333(2):590-5 |
| abstractText | In a previous study, we used mouse zygotes as recipients of mtDNA with a large-scale deletion mutation (DeltamtDNA) and generated respiration-deficient mice (mito-mice) carrying DeltamtDNA. In this study, we used mouse ES cells as recipients of DeltamtDNA, and generated mito-mice with DeltamtDNA only when the ES cells carried 17% DeltamtDNA. No chimera mice or their F(1) progenies were obtained from ES cells carrying more than 61% DeltamtDNA. These observations suggest that respiratory defects of ES cells inhibit their normal differentiation into chimera mice and mito-mice, and that ES cells are more effective than zygotes for generation of mito-mice carrying mtDNAs without significant pathogenic mutations. |
