| Primary Identifier | MGI:5521199 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ndufaf2 |
| Transmission | Germline | Strain of Origin | 129S/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to insert loxP sites around exon 2 and a neo cassette while inserting an inverted exon 2 with multiple stop codons downstream of the floxed sequence. Cre-mediated recombination removed the exon 2 and neo cassette resulting in the placement of the mutant exon 2 in-frame with the remainder of the Ndufaf2 gene. Western blot analysis confirmed the absence of protein expression in skin fibroblasts. |
