| Primary Identifier | MGI:3779038 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Tg(tetO-Cxcl1,-lacZ)19Weik |
| Strain of Origin | (C57BL/6J X DBA/2)F2 | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The construct encodes a bidirectional reporter transgene. The cDNA of the chemokine KC (Cxcl1, from +2 to +140) was cloned into the pBI-G vector (Clontech). The vector contains a tetracycline responsive element consisting of seven copies of the tet-operator (tetO) that is between 2 CMV minimal promoters which are silent in absence of an activator (tTA or rtTA) sequence. When activated, one promoter drives expression of Cxcl1 while the other promoter drives expression of a beta-galactosidase reporter gene. |
