| Primary Identifier | MGI:3784952 | Allele Type | Gene trapped |
| Attribute String | Hypomorph, Reporter | Gene | Zfp148 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Tetraploid complementation analysis of normal appearing embryos at E9.5 and E10.5 reveals grossly normal vasculature and closed neural tubes. Abundant, normal appearing erythrocytes are recovered from E10.5 complementation embryos indicating relatively normal primitive erythropoiesis occurs in absence of Zfp148. No live embryos are observed at E12.5-13.5. |
| molecularNote | A gene trap vector containing a beta-geo cassette was inserted into intron 4 between exon 4 and exon 5. The insertion creates a 163 KDa fusion protein that contains the N-terminus and acidic domain to the beta-galactosidase and neo coding regions. The presence of the fusion protein was confirmed by western blot analysis. This allele is also a reporter allele. |
