| Primary Identifier | MGI:3793642 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(H2-Ea*deltaX)16Dim |
| Strain of Origin | (C57BL/6 x SJL)F2 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | A 9 kb fragment of the H2-Ea(k) from the A/J mouse (clone lambdaA/J p34.13) was modified at 3 positions creating the WE32 construct (modified H2-Ea). This was used as the basis for a mutant H2-Ea gene having a deletion of the X box (from position -93 to -80). The resulting 8.2 kb transgenic construct WEdeltaX21 was injected into (C57BL/6 x SJL)F2 embryo pronuclei. Multiple lines - 5. 16, 23, and 24, were derived, and 16 was donated to The Jackson Laboratory. S1 nuclease mapping shows that H2-Ea mRNA is expressed in the spleen but initiation of most transcripts is aberrant, occurring at scattered positions throughout the 5' end. |
