| Primary Identifier | MGI:3793788 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Eno2-Scn2a1*)Q54Mm |
| Strain of Origin | (C57BL/6J x SJL/J)F2 | Is Recombinase | false |
| Is Wild Type | false |
| description | Phenotypic Similarity to Human Syndrome: Epilepsy (J:136510) |
| molecularNote | A 4 kb promoter fragment from the rat NSE gene that includes 2.8 kb of 5'-flanking sequence, exon 1, intron 1 and 6 bp of exon 215 was used to drive expression of a mutant form of Scn2a1 containing a replacement of residues 879 to 881 (glycine-alanine-leucine) of the wild-type channel with three glutamine residues in the encoded mutant protein. These mutations are in the S4/S5 cytoplasmic linker of domain 2. In addition, a FLAG epitope is located immediately downstream of the initiator methionine. RT-PCR of RNA from dissected brain regions demonstrated that the transgene is widely expressed. No expression in other tissues was detected except for a low level in heart and spleen. |
