Primary Identifier | MGI:3800229 | Allele Type | Gene trapped |
Attribute String | Null/knockout, Reporter | Gene | Rnf8 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The gene trap vector is inserted after exon 4, disrupting proper transcription of the gene and generating a fusion product of beta-geo and the N-terminal part of the encoded protein without its C-terminal RING domain which is critical for function. The absence of protein expression was confirmed by western blot analysis on thymocyte extracts. |