| First Author | Yamauchi T | Year | 2008 |
| Journal | EMBO J | Volume | 27 |
| Issue | 13 | Pages | 1852-62 |
| PubMed ID | 18548008 | Mgi Jnum | J:137631 |
| Mgi Id | MGI:3801372 | Doi | 10.1038/emboj.2008.118 |
| Citation | Yamauchi T, et al. (2008) A B-Myb complex containing clathrin and filamin is required for mitotic spindle function. EMBO J 27(13):1852-62 |
| abstractText | B-Myb is one member of the vertebrate Myb family of transcription factors and is ubiquitously expressed. B-Myb activates transcription of a group of genes required for the G2/M cell cycle transition by forming the dREAM/Myb-MuvB-like complex, which was originally identified in Drosophila. Mutants of zebrafish B-myb and Drosophila myb exhibit defects in cell cycle progression and genome instability. Although the genome instability caused by a loss of B-Myb has been speculated to be due to abnormal cell cycle progression, the precise mechanism remains unknown. Here, we have purified a B-Myb complex containing clathrin and filamin (Myb-Clafi complex). This complex is required for normal localization of clathrin at the mitotic spindle, which was previously reported to stabilize kinetochore fibres. The Myb-Clafi complex is not tightly associated with the mitotic spindles, suggesting that this complex ferries clathrin to the mitotic spindles. Thus, identification of the Myb-Clafi complex reveals a previously unrecognized function of B-Myb that may contribute to its role in chromosome stability, possibly, tumour suppression. |
