| Primary Identifier | MGI:3803587 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Hras |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | This mutation was generated by re-targeting R1 ES cells in which Hras had been targeted to create Hrastm1Xbr. (J:137729) |
| molecularNote | In ES cells containing Hras1tm1Xbr, a floxed hygro STOP cassette was inserted upstream of coding exon 1. In addition, a G to T substitutions was created at the second base of codon 12 of exon 1. This results in an amino acid substitution of valine for glycine. Germ line, cre-mediated recombination was used to remove the hygro STOP cassette. The final allele contains the G12V mutation and the IRES-beta-geo cassette. |
