| Primary Identifier | MGI:3806307 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase | Gene | Tg(Kap-icre)29066/2Sig |
| Strain of Origin | C57BL/6J x SJL | Induced With | androgen |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The Kap promoter and the distal regulatory sequences of the human angiotensinogen gene (AGT), which together confer androgen responsiveness, is used to drive expression of androgen-inducible improved Cre (iCre) in the kidney. The icre cDNA was cloned downstream of the Kap promoter (nucleotides -1542 through -1 relative to the transcription initiation site) into the NotI site of the pKAP2 vector. This is followed by the last 42 bp of human AGT exon 2 through exon 5, which lost their coding potential owing to the lack of the first 817 bp of exon 2 that included the ATG start site. Twenty-six founders were created of which only line 29066/2 expressed cre in male kidneys with some expression in the brain, liver, and heart. |
