| First Author | Legname G | Year | 2000 |
| Journal | Immunity | Volume | 12 |
| Issue | 5 | Pages | 537-46 |
| PubMed ID | 10843386 | Mgi Jnum | J:110527 |
| Mgi Id | MGI:3640449 | Doi | 10.1016/s1074-7613(00)80205-8 |
| Citation | Legname G, et al. (2000) Inducible expression of a p56Lck transgene reveals a central role for Lck in the differentiation of CD4 SP thymocytes. Immunity 12(5):537-46 |
| abstractText | The T lymphocyte-specific protein tyrosine kinase p56lck (Lck) is an essential component of the TCR-mediated signal transduction complex. Lck knockout mice have reduced numbers of double-positive thymocytes and very few mature single-positive cells, particularly of the CD4 lineage. Here we demonstrate the ability of a tetracycline-based tissue-specific inducible Lck transgene to restore expansion of early thymocytes and maturation of single-positive cells in Lckneg mice upon induction with doxycycline. Restoration of Lck expression is particularly important for positive selection to the CD4+ lineage but has a lesser impact on selection to the CD8+ lineage, suggesting activation of Lck is an important component of the signals involved in lineage choice during thymic differentiation. |
