| First Author | Jones MJ | Year | 2009 |
| Journal | Dev Biol | Volume | 336 |
| Issue | 1 | Pages | 42-52 |
| PubMed ID | 19778534 | Mgi Jnum | J:154092 |
| Mgi Id | MGI:4367226 | Doi | 10.1016/j.ydbio.2009.09.023 |
| Citation | Jones MJ, et al. (2009) An imprinted GFP insertion reveals long-range epigenetic regulation in embryonic lineages. Dev Biol 336(1):42-52 |
| abstractText | Imprinted genes are often grouped in clusters at defined chromosomal locations. Long-range regulatory effects are implicated in the control of imprinting and these could be co-opted in the emergence of novel imprinted genes during evolution. We present a detailed analysis of a novel imprinted GFP mouse line. Tel7KI is a new insertion allele near the Ins2 locus within a cluster of imprinted genes on distal mouse Chr7. The GFP reporter becomes regulated by the host domain in two notable fashions. First, transcription of GFP is imprinted and active exclusively from the maternally inherited allele in the embryo. Second, the expressed maternal allele is subject to position effects reflecting a distinct pattern of expression. The GFP reporter acquires silencing DNA methylation marks on the paternal allele after fertilization. This imprinting is not acquired in the placenta, where GFP is active from both parental alleles, demonstrating key epigenetic differences between embryonic and extraembryonic lineages. Our analysis shows that imprinted clusters can provide environments conducive to the acquisition of imprinting upon novel inserted transcriptional units. The Tel7KI line offers new powerful avenues to explore both genetic and environmental factors implicated in the acquisition and maintenance of imprinted transcription in mammals. |
