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Allele : Gt(ROSA)26Sor<tm3(CAG-EYFP)Hze> gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Hongkui Zeng
Primary Identifier  MGI:3809521 Allele Type  Targeted
Attribute String  Conditional ready, Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  (129S6/SvEvTac x C57BL/6NCrl)F1
Is Recombinase  false Is Wild Type  false
molecularNote  The Rosa-CAG-LSL-EYFP-WPRE targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), an enhanced yellow fluorescent protein sequence (from pcDNA6.2/N-YFP-DEST), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. This entire construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
  • mutations:
  • Insertion
  • synonyms:
  • R26<LSL-YFP>,
  • R26<LSL-YFP>,
  • Ai3,
  • Rosa-CAG-LSL-EYFP-WPRE,
  • Ai3,
  • Rosa-CAG-LSL-EYFP-WPRE
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Trail: Allele

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0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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Trail: Allele




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