| First Author | Klimas A | Year | 2023 |
| Journal | Nat Biotechnol | Volume | 41 |
| Issue | 6 | Pages | 858-869 |
| PubMed ID | 36593399 | Mgi Jnum | J:359588 |
| Mgi Id | MGI:7788567 | Doi | 10.1038/s41587-022-01546-1 |
| Citation | Klimas A, et al. (2023) Magnify is a universal molecular anchoring strategy for expansion microscopy. Nat Biotechnol 41(6):858-869 |
| abstractText | Expansion microscopy enables nanoimaging with conventional microscopes by physically and isotropically magnifying preserved biological specimens embedded in a crosslinked water-swellable hydrogel. Current expansion microscopy protocols require prior treatment with reactive anchoring chemicals to link specific labels and biomolecule classes to the gel. We describe a strategy called Magnify, which uses a mechanically sturdy gel that retains nucleic acids, proteins and lipids without the need for a separate anchoring step. Magnify expands biological specimens up to 11 times and facilitates imaging of cells and tissues with effectively around 25-nm resolution using a diffraction-limited objective lens of about 280 nm on conventional optical microscopes or with around 15 nm effective resolution if combined with super-resolution optical fluctuation imaging. We demonstrate Magnify on a broad range of biological specimens, providing insight into nanoscopic subcellular structures, including synaptic proteins from mouse brain, podocyte foot processes in formalin-fixed paraffin-embedded human kidney and defects in cilia and basal bodies in drug-treated human lung organoids. |
