| First Author | Viant C | Year | 2020 |
| Journal | Cell | Volume | 183 |
| Issue | 5 | Pages | 1298-1311.e11 |
| PubMed ID | 33125897 | Mgi Jnum | J:298615 |
| Mgi Id | MGI:6477770 | Doi | 10.1016/j.cell.2020.09.063 |
| Citation | Viant C, et al. (2020) Antibody Affinity Shapes the Choice between Memory and Germinal Center B Cell Fates. Cell 183(5):1298-1311.e11 |
| abstractText | Immunological memory is required for protection against repeated infections and is the basis of all effective vaccines. Antibodies produced by memory B cells play an essential role in many of these responses. We have combined lineage tracing with antibody cloning from single B cells to examine the role of affinity in B cell selection into germinal centers (GCs) and the memory B cell compartment in mice immunized with an HIV-1 antigen. We find that contemporaneously developing memory and GC B cells differ in their affinity for antigen throughout the immune response. Whereas GC cells and their precursors are enriched in antigen binding, memory B cells are not. Thus, the polyclonal memory B cell compartment is composed of B cells that were activated during the immune response but whose antigen binding affinity failed to support further clonal expansion in the GC. |
