| First Author | Luo F | Year | 2023 |
| Journal | Exp Neurol | Volume | 359 |
| Pages | 114250 | PubMed ID | 36240882 |
| Mgi Jnum | J:331661 | Mgi Id | MGI:7380104 |
| Doi | 10.1016/j.expneurol.2022.114250 | Citation | Luo F, et al. (2022) T-type Ca(2+) channels and inward rectifier K(+) channels contribute to the orexin-induced facilitation of GABAergic transmission onto pyramidal neurons in the prefrontal cortex of juvenile mice. Exp Neurol 359:114250 |
| abstractText | Orexin is a neuropeptide restrictedly synthesized in the hypothalamus, but extensively modulates the whole brain region activity including prefrontal cortex (PFC), and involved in the pathophysiology of psychiatric disorders. GABAergic interneurons in the mPFC are a promising pharmacological target for developing antidepressant therapies. Here, we examined the effects of the orexin on GABAergic transmission onto pyramidal neurons in the deep layers of the mPFC. We found that bath application of orexin dose-dependently increased the amplitude of evoked IPSCs (eIPSCs). Orexin increased the frequency but not the amplitude of miniature IPSCs (mIPSCs). Ca(2+) influx through T-type voltage-gated Ca(2+) channels is required for orexin-induced increases in GABA release. We also found orexin increases GABA release probability and the number of releasable vesicles. Orexin depolarizes somatostatin (Sst) interneurons without effects on the firing rate of action potentials (APs) of Sst interneurons. Orexin-induced depolarization of Sst interneurons is independent of extracellular Na(+), Ca(2+) and T-type Ca(2+) channels, but requires inward rectifier K(+) channels (Kirs). The present study suggests that orexin enhances GABAergic transmission onto mPFC pyramidal neurons through inhibiting Kirs on Sst interneurons, which further depolarizes interneurons leading to increase in Ca(2+) influx via T-type Ca(2+) channels. Our results may provide a cellular and molecular mechanism that helps explain the physiological functions of orexin in the brain. |
