| First Author | Farzinpour Z | Year | 2022 |
| Journal | Front Cell Neurosci | Volume | 16 |
| Pages | 898346 | PubMed ID | 35910255 |
| Mgi Jnum | J:327153 | Mgi Id | MGI:7327792 |
| Doi | 10.3389/fncel.2022.898346 | Citation | Farzinpour Z, et al. (2022) Microglial Engulfment of Spines in the Ventral Zona Incerta Regulates Anxiety-Like Behaviors in a Mouse Model of Acute Pain. Front Cell Neurosci 16:898346 |
| abstractText | Although activation of microglial cells is critical in developing brain disorders, their role in anxiety-like behaviors in pain is still vague. This study indicates that alteration of microglia's neuronal spine engulfment capacity in ventral zona incerta (ZI V ) leads to significant pain and anxiety-like behaviors in mice 1-day post-injection of Complete Freud's Adjuvant (CFA1D). Performing whole-cell patch-clamp recordings in GABAergic neurons in the ZI V (ZI V (GABA) ) in brain slices, we observed decreased activity in ZIv (GABA) and reduced frequency of the miniature excitatory postsynaptic currents (mEPSCs) in ZI V (GABA) of CFA1D mice compared with the saline1D mice. Besides, chemogenetic activation of ZI V (GABA) significantly relieved pain and anxiety-like behaviors in CFA1D mice. Conversely, in naive mice, chemogenetic inhibition of ZI V (GABA) induced pain and anxiety-like behaviors. Interestingly, we found changes in the density and morphology of ZI V (Microglia) and increased microglial engulfment of spines in ZI V of CFA1D mice. Furthermore, pain sensitization and anxiety-like behaviors were reversed when the ZI V (Microglia) of CFA1D-treated mice were chemically inhibited by intra-ZI V minocycline injection, accompanied by the recovery of decreased ZI V (GABA) excitability. Conclusively, our results provide novel insights that dysregulation of microglial engulfment capacity encodes maladaptation of ZI V (GABA) , thus promoting the development of anxiety-like behaviors in acute pain. |
