| First Author | Kubota M | Year | 2021 |
| Journal | STAR Protoc | Volume | 2 |
| Issue | 3 | Pages | 100645 |
| PubMed ID | 34278332 | Mgi Jnum | J:350630 |
| Mgi Id | MGI:6728258 | Doi | 10.1016/j.xpro.2021.100645 |
| Citation | Kubota M, et al. (2021) Murine cochlear cell sorting and cell-type-specific organoid culture. STAR Protoc 2(3):100645 |
| abstractText | Neonatal mouse cochlear duct cells can proliferate and grow in vitro into inner ear organoids. Distinctive cochlear duct cell types have different organoid formation capacities. Here, we provide a flow cytometric cell-sorting method that allows the subsequent culture of individual cochlear cell populations. For the efficient culture of the sorted cells, we provide protocols for growing free-floating inner ear organoids, the adherence of organoids to a substrate, and the expansion of organoid-derived inner ear colonies. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2021). |
