| First Author | Daniel JA | Year | 2017 |
| Journal | Elife | Volume | 6 |
| PubMed ID | 28598330 | Mgi Jnum | J:257087 |
| Mgi Id | MGI:6116913 | Doi | 10.7554/eLife.26338 |
| Citation | Daniel JA, et al. (2017) Analysis of SUMO1-conjugation at synapses. Elife 6:e26338 |
| abstractText | SUMO1-conjugation of proteins at neuronal synapses is considered to be a major post-translational regulatory process in nerve cell and synapse function, but the published evidence for SUMO1-conjugation at synapses is contradictory. We employed multiple genetic mouse models for stringently controlled biochemical and immunostaining analyses of synaptic SUMO1-conjugation. By using a knock-in reporter mouse line expressing tagged SUMO1, we could not detect SUMO1-conjugation of seven previously proposed synaptic SUMO1-targets in the brain. Further, immunostaining of cultured neurons from wild-type and SUMO1 knock-out mice showed that anti-SUMO1 immunolabelling at synapses is non-specific. Our findings indicate that SUMO1-conjugation of synaptic proteins does not occur or is extremely rare and hence not detectable using current methodology. Based on our data, we discuss a set of experimental strategies and minimal consensus criteria for the validation of SUMOylation that can be applied to any SUMOylation substrate and SUMO isoform. |
