| First Author | Tang H | Year | 2008 |
| Journal | Transgenic Res | Volume | 17 |
| Issue | 4 | Pages | 599-608 |
| PubMed ID | 18288583 | Mgi Jnum | J:140275 |
| Mgi Id | MGI:3813193 | Doi | 10.1007/s11248-008-9170-y |
| Citation | Tang H, et al. (2008) Characterization of Ayu17-449 gene expression and resultant kidney pathology in a knockout mouse model. Transgenic Res 17(4):599-608 |
| abstractText | The gene trap technique is a powerful approach for characterizing and mutating genes in the mouse. We used this method to identify a mouse gene of unknown function and to establish a mutant mouse line. We subsequently identified one gene, denoted Ayu17-449, on mouse chromosome 3 that comprised 14 exons encoding 1920 amino acids with a granin motif in its N-terminal sequence. In adult mice, this gene was highly expressed in the brain, heart, lung, muscle, stomach, and kidney. The insertion of a trap vector into the second intron of this gene resulted in the null mutation. Homozygous mice for these mutation died by 1 day after birth. Mutant mice showed a loss of acidic granules in the proximal convoluted tubules of the kidney. Our data demonstrates that Ayu17-449 is important for mouse survival. |
