| Primary Identifier | MGI:3817448 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Raf1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed neo cassette was inserted in intron 13 and a D486A mutation was inserted in exon 14 via homologous recombination. The neo cassette was subsequently removed via in vivo Cre mediated recombination. The D486A mutation creates a kinase inactive protein. Western blot analysis detected a greater than 50 fold reduction in protein expression in homozygous embryos and MEFs. |
