| First Author | Yang G | Year | 2018 |
| Journal | Biochim Biophys Acta | Volume | 1863 |
| Issue | 2 | Pages | 165-176 |
| PubMed ID | 29191638 | Mgi Jnum | J:257704 |
| Mgi Id | MGI:6119129 | Doi | 10.1016/j.bbalip.2017.11.008 |
| Citation | Yang G, et al. (2018) Cystathionine gamma-lyase/hydrogen sulfide system is essential for adipogenesis and fat mass accumulation in mice. Biochim Biophys Acta 1863(2):165-176 |
| abstractText | Hydrogen sulfide (H2S) has been recognized as an important gasotransmitter analogous to nitric oxide and carbon monoxide. Cystathionine gamma-lyase (CSE)-derived H2S is implicated in the regulation of insulin resistance and glucose metabolism, but the involvement of CSE/H2S system in energy homeostasis and fat mass has not been extensively explored. In this study, a potential functional role of the CSE/H2S system in in vitro adipocyte differentiation and in vivo adipogenesis and the underlying mechanism was investigated. CSE expression and H2S production were increased during adipocyte differentiation, and that the pattern of CSE mRNA expression was similar to that of CCAAT/enhancer-binding protein (C/EBP) beta and delta, two key regulators for adipogenesis. C/EBPbeta and gamma bind to the CCAAT box in CSE promoter and stimulate CSE gene transcription. H2S induced PPARgamma transactivation activity by S-sulfhydrating all the cysteine residues in the DNA binding domain and stimulated adipogenesis. High fat diet-induced fat mass was lost in CSE deficient mice, and exogenously applied H2S promoted fat mass accumulation in fruit flies. In conclusion, CSE/H2S system is essential for adipogenesis and fat mass accumulation through enhancement of PPARgamma function in adipocytes. This study suggests that the CSE/H2S system is involved in the pathogenesis of obesity in mice. |
