| First Author | Zhao K | Year | 2014 |
| Journal | EMBO Rep | Volume | 15 |
| Issue | 7 | Pages | 792-800 |
| PubMed ID | 24778456 | Mgi Jnum | J:216526 |
| Mgi Id | MGI:5608948 | Doi | 10.1002/embr.201338213 |
| Citation | Zhao K, et al. (2014) S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO Rep 15(7):792-800 |
| abstractText | The repair of DNA damage is fundamental to normal cell development and replication. Hydrogen sulfide (H2S) is a novel gasotransmitter that has been reported to protect cellular aging. Here, we show that H2S attenuates DNA damage in human endothelial cells and fibroblasts by S-sulfhydrating MEK1 at cysteine 341, which leads to PARP-1 activation. H2S-induced MEK1 S-sulfhydration facilitates the translocation of phosphorylated ERK1/2 into nucleus, where it activates PARP-1 through direct interaction. Mutation of MEK1 cysteine 341 inhibits ERK phosphorylation and PARP-1 activation. In the presence of H2S, activated PARP-1 recruits XRCC1 and DNA ligase III to DNA breaks to mediate DNA damage repair, and cells are protected from senescence. |
