| Primary Identifier | MGI:3815158 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cidec |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line EB3/5 was derived from ES cell line E14TG2a. EB3/5 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:140863 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | Exons 1 and 2 were replaced with a neo cassette. The absence of protein expression was confirmed by western blot analysis on white adipose tissue extracts. ES cells used to create this allele were EB3/5. |
