| First Author | Nishimoto Y | Year | 2017 |
| Journal | J Biol Chem | Volume | 292 |
| Issue | 26 | Pages | 10824-10834 |
| PubMed ID | 28490632 | Mgi Jnum | J:246420 |
| Mgi Id | MGI:5916423 | Doi | 10.1074/jbc.M116.768820 |
| Citation | Nishimoto Y, et al. (2017) Cell death-inducing DNA fragmentation factor A-like effector A and fat-specific protein 27beta coordinately control lipid droplet size in brown adipocytes. J Biol Chem 292(26):10824-10834 |
| abstractText | Adipose tissue stores neutral lipids and is a major metabolic organ involved in regulating whole-body energy homeostasis. Triacylglycerol is stored as unilocular large lipid droplets (LDs) in white adipocytes and as multilocular small LDs in brown adipocytes. Proteins of the cell death-inducing DNA fragmentation factor A-like effector (Cide) family include CideA, CideB, and fat-specific protein of 27 (FSP27). Of these, FSP27 has been shown to play a crucial role in the formation of unilocular large LDs in white adipocytes. However, the mechanisms by which brown adipocytes store small and multilocular LDs remain unclear. An FSP27 isoform, FSP27beta, was recently identified. We herein report that CideA and FSP27beta are mainly expressed in brown adipose tissue and that FSP27beta overexpression inhibits CideA-induced LD enlargements in a dose-dependent manner in COS cells. Furthermore, RNAi-mediated FSP27beta depletion resulted in enlarged LDs in HB2 adipocytes, which possess the characteristics of brown adipocytes. Brown adipocytes in FSP27-knock-out mice that express CideA, but not FSP27beta, had larger and fewer LDs. Moreover, we confirmed that FSP27beta and CideA form a complex in brown adipose tissue. Our results suggest that FSP27beta negatively regulates CideA-promoted enlargement of LD size in brown adipocytes. FSP27beta appears to be responsible for the formation of small and multilocular LDs in brown adipose tissue, a morphology facilitating free fatty acid transport to mitochondria adjacent to LDs for oxidation in brown adipocytes. |
