| Primary Identifier | MGI:3815067 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Cthrc1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:140886 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | EB3 ES cells were targeted with a vector containing a lacZ and PGK-neo cassette, removing exon 1, including the translation initiation codon. Absence of transcript was confirmed by Northern blot analysis. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14Tg2a ES cells with an IRESBSDpA selection cassette. |
