| Primary Identifier | MGI:3818438 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tgfbr3 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 of this locus encodes the N terminus, including a portion of the extracellular ligand binding domain. A floxed neo-cassette was placed upstream of exon 3 and a single loxP site was placed downstream of exon 3. Mice carrying the targeted mutation in the germline were crossed with cre-transgenic mice to remove exon 3 and the neo-cassette, leaving in its place a single loxP site. Ligand crosslinking with 125I-TGFbeta to embryonic fibroblasts isolated from homozygotes confirms loss of protein expression. |
