| First Author | Emperador-Melero J | Year | 2024 |
| Journal | Nat Neurosci | Volume | 27 |
| Issue | 9 | Pages | 1680-1694 |
| PubMed ID | 39160372 | Mgi Jnum | J:358058 |
| Mgi Id | MGI:7764564 | Doi | 10.1038/s41593-024-01720-5 |
| Citation | Emperador-Melero J, et al. (2024) Distinct active zone protein machineries mediate Ca(2+) channel clustering and vesicle priming at hippocampal synapses. Nat Neurosci 27(9):1680-1694 |
| abstractText | Action potentials trigger neurotransmitter release at the presynaptic active zone with spatiotemporal precision. This is supported by protein machinery that mediates synaptic vesicle priming and clustering of Ca(V)2 Ca(2+) channels nearby. One model posits that scaffolding proteins directly tether vesicles to Ca(V)2s; however, here we find that at mouse hippocampal synapses, Ca(V)2 clustering and vesicle priming are executed by separate machineries. Ca(V)2 nanoclusters are positioned at variable distances from those of the priming protein Munc13. The active zone organizer RIM anchors both proteins but distinct interaction motifs independently execute these functions. In transfected cells, Liprin-alpha and RIM form co-assemblies that are separate from Ca(V)2-organizing complexes. At synapses, Liprin-alpha1-Liprin-alpha4 knockout impairs vesicle priming but not Ca(V)2 clustering. The cell adhesion protein PTPsigma recruits Liprin-alpha, RIM and Munc13 into priming complexes without co-clustering Ca(V)2s. We conclude that active zones consist of distinct machineries to organize Ca(V)2s and prime vesicles, and Liprin-alpha and PTPsigma specifically support priming site assembly. |
