| Primary Identifier | MGI:3823016 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Etnk2 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene locus was inactivated by inserting the enhanced green fluorescence protein (GFP) coding sequence in-frame into the ATG-start site of Eki2, replacing part of exon 1 and exon 2. A loxP-flanked neo selection cassette was included downstream of the GFP cassette and was subsequently removed by crossing mice with CMV-cre transgenic mice. Gene inactivation was confirmed by quantitative PCR of testis RNA. GFP expression was observed in the testis of E13.5 embryos and adult males. |
