| Primary Identifier | MGI:3829069 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Apc |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP-flanked puromycin resistance cassette was introduced to an intron 5' of the first coding exon. Cre-mediated recombination was used to excise the floxed region, leaving a single loxP site. Genomic regions 3' of the gene were then targeted with a loxP-FRT-neomycin-FRT cassette. FLPe-mediated recombination was done to excise the neomycin resistance gene leaving loxP and FRT sites 3' of the gene. |
