| First Author | Zhou K | Year | 2024 |
| Journal | Brain Behav Immun | Volume | 123 |
| Pages | 57-63 | PubMed ID | 39218233 |
| Mgi Jnum | J:354014 | Mgi Id | MGI:7717241 |
| Doi | 10.1016/j.bbi.2024.08.055 | Citation | Zhou K, et al. (2024) Microglia depletion and repopulation do not alter the effects of cranial irradiation on hippocampal neurogenesis. Brain Behav Immun 123:57-63 |
| abstractText | Cranial radiotherapy can cause lifelong cognitive complications in childhood brain tumor survivors, and reduced hippocampal neurogenesis is hypothesized to contribute to this. Following irradiation (IR), microglia clear dead neural progenitors and give rise to a neuroinflammatory microenvironment, which promotes a switch in surviving progenitors from neuronal to glial differentiation. Recently, depletion and repopulation of microglia were shown to promote neurogenesis and ameliorate cognitive deficits in various brain injury models. In this study, we utilized the Cx3cr1(CreERt2-YFP/+)Rosa26(DTA)(/+) transgenic mouse model to deplete microglia in the juvenile mouse brain before subjecting them to whole-brain IR and investigated the short- and long-term effects on hippocampal neurogenesis. Within the initial 24 h after IR, the absence of microglia led to an accumulation of dead cells in the subgranular zone, and 50-fold higher levels of the chemokine C-C motif ligand 2 (CCL2) in sham brains and 7-fold higher levels after IR. The absence of microglia, and the subsequent repopulation within 10 days, did neither affect the loss of proliferating or doublecortin-positive cells, nor the reduced growth of the granule cell layer. Our results argue against a role for a pro-inflammatory microenvironment in the dysregulation of hippocampal neurogenesis and suggest that the observed reduction of neurogenesis was solely due to IR. |
