| Primary Identifier | MGI:3829430 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Pthlh |
| Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 was replaced with a LacZ reporter cassette. The lacZ cassette encoded the beta-gal coding region preceded by stop codons (TAG) in all three reading frames. The construct also uses a polyA sequence to replace the untranslated region of the gene locus, which contains AU-rich instability sequences. Beta-gal activity was detected in numerous tissues of heterozygous embryos including the mammary bud, inner ear, nasal epithelium, and in the endochondral skeleton. Beta-gal activity was also observed in tendon insertion sites of adult mice and within descrete populations of chondrocytes. |
