| Primary Identifier | MGI:3832386 | Allele Type | Targeted |
| Attribute String | Conditional ready, Humanized sequence, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | DNA encoding influenza virus hemagglutinin A- (HA-) tagged human hypoxia-inducible factor 2 alpha (HIF2alpha) with nucleotide substitutions leading to replacement of proline by alanine at amino acid positions 405 and 531 (P405A;P531A), preceded by a floxed neo-STOP cassette, was inserted into the targeted locus. The STOP sequence must be removed by Cre-mediated recombination to allow for expression of the mutated coding sequence. The amino acid substitutions prevent oxygen-dependent prolyl hydroxylation at a.a. position 405 or 531, which is required for binding by von Hippel-Lindau tumor suppressor (VHL). |
