| Primary Identifier | MGI:3830630 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | (B6.129P2-Hprt1<b-m3>/J x 129S-Gt(ROSA)26Sor<tm1Sor>/J)F1 |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Pleiades Promoter Project |
| description | Germ line transmission of mutant cell line mEMS1615 has been established. |
| molecularNote | The Ple151-EGFP transgene (pEMS1172) was designed with a Ple151 minipromoter (OLIG1-D; a combination of four regulatory sequences from the human oligodendrocyte transcription factor 1 (OLIG1) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome. The promoter/regulatory regions of the human oligodendrocyte transcription factor 1 (OLIG1) gene direct expression of enhanced green fluorescent protein (EGFP) to neural white matter glia and oligodendroglia. EGFP expression (via anti-GFP immunocytochemistry) is observed throughout cortical and subcortical regions of the brain. Within the cortex, individual cell bodies are distinctly labeled and "puffy" processes surround these cells. In contrast to the predicted expression of OLIG1, EGFP expression does not appear to be enriched in white matter tracts or in the corpus callosum. |
