| First Author | Luuk H | Year | 2012 |
| Journal | Biochem Biophys Res Commun | Volume | 424 |
| Issue | 4 | Pages | 717-23 |
| PubMed ID | 22800759 | Mgi Jnum | J:186231 |
| Mgi Id | MGI:5431244 | Doi | 10.1016/j.bbrc.2012.07.017 |
| Citation | Luuk H, et al. (2012) Circadian rhythms and food anticipatory behavior in Wfs1-deficient mice. Biochem Biophys Res Commun 424(4):717-23 |
| abstractText | The dorsomedial hypothalamic nucleus (DMH) has been proposed as a candidate for the neural substrate of a food-entrainable oscillator. The existence of a food-entrainable oscillator in the mammalian nervous system was inferred previously from restricted feeding-induced behavioral rhythmicity in rodents with suprachiasmatic nucleus lesions. In the present study, we have characterized the circadian rhythmicity of behavior in Wfs1-deficient mice during ad libitum and restricted feeding. Based on the expression of Wfs1 protein in the DMH it was hypothesized that Wfs1-deficient mice will display reduced or otherwise altered food anticipatory activity. Wfs1 immunoreactivity in DMH was found almost exclusively in the compact part. Restricted feeding induced c-Fos immunoreactivity primarily in the ventral and lateral aspects of DMH and it was similar in both genotypes. Wfs1-deficiency resulted in significantly lower body weight and reduced wheel-running activity. Circadian rhythmicity of behavior was normal in Wfs1-deficient mice under ad libitum feeding apart from elongated free-running period in constant light. The amount of food anticipatory activity induced by restricted feeding was not significantly different between the genotypes. Present results indicate that the effects of Wfs1-deficiency on behavioral rhythmicity are subtle suggesting that Wfs1 is not a major player in the neural networks responsible for circadian rhythmicity of behavior. |
