| Primary Identifier | MGI:3838714 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Igh-7 |
| Transmission | Germline | Strain of Origin | BALB/c |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A point mutation was targeted to the third codon of exon 6 to introduce a stop codon and a frameshift mutation to the portion of the gene that encodes the cytoplasmic tail. LoxP sites were also inserted upstream of exon 5 and downstream of the mutated exon 6. A neomycin/tk selection cassette followed by a third loxP site was inserted further downstream. Transient expression of cre recombinase removed the selection cassette while leaving the mutated exon 6 in place. Correct targeting and recombination were confirmed by Southern blotting. This allele was predicted to encode an IgE heavy chain with a cytoplasmic tail only 3 amino acids long (KVK) that was identical to the cytoplasmic domain of mIgM and mIgD. |
