| Primary Identifier | MGI:3838345 | Allele Type | Gene trapped |
| Attribute String | Null/knockout, Reporter | Gene | Azin1 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The gene trap vector, pU-17, integrated at a single site in the mutant cell line. This vector is composed of a mouse En2 splicing acceptor, a lox71 site, beta galactosidase, neomycin, loxP, a poly A site, and lox2272. The integrated trapping vector was found to have a 522 bp deletion at its 5' terminus. Vector insertion was in intron 2 about 9.295 kb upstream of exon3 in which the translational start codon is located. Northern blot analysis demonstrated that gene expression was inhibited in heterozygotes and strongly suppressed in homozygotes. |
