| Primary Identifier | MGI:3843456 | Allele Type | Targeted |
| Attribute String | Humanized sequence | Gene | Avp |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A floxed pgk-neo cassette was inserted in intron 2 and a Cys98stop nonsense mutation was inserted in exon 2 via homologous recombination. The floxed neo cassette was then removed by Cre mediated recombination. Immunohistochemical analysis detected expression of a mutant protein in the supraoptic nucleus, the suprachiasmatic nuclei, and the paraventricular nucleus in homozygous and heterozygous mice. This analysis also confirmed the absence of wild-type protein expression in homozygous mice. |
