| Primary Identifier | MGI:3844726 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Dclk2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 2 and a floxed neomycin selection cassette was inserted downstream of the exon. Exon 2 was removed by crossing founder mice with transgenic mice that express cre recombinase in the germline removed exon 2, and led to an out-of-frame mutation and the appearance of a stop codon within exon 3. Immunoblot analysis indicated a reduction in protein expression in heterozygote brain, and absence of protein in homozygote brain. |
