| First Author | Banerjee A | Year | 2018 |
| Journal | J Immunol | Volume | 200 |
| Issue | 1 | Pages | 163-176 |
| PubMed ID | 29187586 | Mgi Jnum | J:253338 |
| Mgi Id | MGI:6108084 | Doi | 10.4049/jimmunol.1700674 |
| Citation | Banerjee A, et al. (2018) Live Attenuated Leishmania donovani Centrin Gene-Deleted Parasites Induce IL-23-Dependent IL-17-Protective Immune Response against Visceral Leishmaniasis in a Murine Model. J Immunol 200(1):163-176 |
| abstractText | No vaccine exists against visceral leishmaniasis. To develop effective vaccines, we have previously reported protective role of live attenuated centrin gene-deleted Leishmania donovani (LdCen(-/-) ) parasites through induction of Th1 type immune response in mice, hamsters, and dogs. In this study, we specifically explored the role of Th17 cells in LdCen(-/-) -induced host protection in mice. Our results showed that compared with wild-type L. donovani infection, LdCen(-/-) parasites induce significantly higher expression of Th17 differentiation cytokines in splenic dendritic cells. There was also induction of IL-17 and its promoting cytokines in total splenocytes and in both CD4 and CD8 T cells following immunization with LdCen(-/-) Upon challenge with wild-type parasites, IL-17 and its differentiating cytokines were significantly higher in LdCen(-/-) -immunized mice compared with nonimmunized mice that resulted in parasite control. Alongside IL-17 induction, we observed induction of IFN-gamma-producing Th1 cells as reported earlier. However, Th17 cells are generated before Th1 cells. Neutralization of either IL-17 or IFN-gamma abrogated LdCen(-/-) -induced host protection further confirming the essential role of Th17 along with Th1 cytokines in host protection. Treatment with recombinant IL-23, which is required for stabilization and maintenance of IL-17, heightened Th17, and Tc17 responses in immunized mice splenocytes. In contrast, Th17 response was absent in immunized IL-23R(-/-) mice that failed to induce protection upon virulent Leishmania challenge suggesting that IL-23 plays an essential role in IL-17-mediated protection by LdCen(-/-) parasites. This study unveiled the role of IL-23-dependent IL-17 induction in LdCen(-/-) parasite-induced immunity and subsequent protection against visceral leishmaniasis. |
