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Publication : Tracking neuronal motility in live murine retinal explants.

First Author  Aghaizu ND Year  2021
Journal  STAR Protoc Volume  2
Issue  4 Pages  101008
PubMed ID  34917982 Mgi Jnum  J:354432
Mgi Id  MGI:7734868 Doi  10.1016/j.xpro.2021.101008
Citation  Aghaizu ND, et al. (2021) Tracking neuronal motility in live murine retinal explants. STAR Protoc 2(4):101008
abstractText  The developing retina undergoes dynamic organizational changes involving significant intra-retinal motility of the encompassing cells. Here, we present a protocol for tracking retinal cell motility in live explanted mouse retinae. Although originally applied to rod and cone photoreceptors, this strategy is applicable to any fluorescently labeled cell in mouse retinae and other similar experimental retinal models. Careful tissue handling is critical for the successful acquisition of high-quality live imaging data. Further instructions for semi-automated in silico data handling are provided. For complete details on the use and execution of this protocol, please refer to Aghaizu et al. (2021).
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