| Primary Identifier | MGI:3849413 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Mecom |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP site was inserted upstream of exon 4 and a floxed neomycin cassette was placed downstream. After targeting and selection, transient expression of cre recombinase removed the neomycin cassette and left exon 4 floxed. Correct targeting was confirmed by Southern blotting. Expression of cre recombinase will remove exon 4, which encodes a zinc finger domain common to the three major protein isoforms of the gene locus. |
