| Primary Identifier | MGI:3850559 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Vcan |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An endoplasmic reticulum retention signal (KDEL) followed by a STOP codon and floxed neo cassette was inserted into exon 7 after codon E749. This exon encodes the GAG-alpha domain. Germ line, cre mediated recombination removed the neo cassette. Quantitative RT-PCR on brain extracts confirmed a 90% reduction in isoform V2 transcript expression and a decline of isoform V0 transcript to 20% (at P0) or 50% (at P30 and P100) of the levels in wild-type mice. Transcript expression of isoform V1 and V3 is unaffected. Immunohistochemistry confirmed the absence of protein expression of isoforms V0 and V2. |
