| First Author | Crozat E | Year | 2025 |
| Journal | Commun Biol | Volume | 8 |
| Issue | 1 | Pages | 1233 |
| PubMed ID | 40817183 | Mgi Jnum | J:376458 |
| Mgi Id | MGI:8239198 | Doi | 10.1038/s42003-025-08559-x |
| Citation | Crozat E, et al. (2025) Cerebrospinal fluid-contacting neurons are sensory neurons with uniform morphological and region-specific electrophysiological properties in the mouse spinal cord. Commun Biol 8(1):1233 |
| abstractText | Cerebrospinal Fluid-contacting neurons (CSF-cNs) are GABAergic bipolar neurons found, in contact with the cerebrospinal fluid, along the vertebrate medullo-spinal central canal. They express Polycystin Kidney Disease 2-Like 1 channels (PKD2L1), members of the Transient Receptor Potential superfamily, and were shown to modulate motor activity and therefore suggested to act as a novel sensory system. However, in mice, they remain largely uncharacterized and it is crucial to comprehensively characterize their morphological and electrophysiological properties to determine whether they form a homogenous neuronal population and understand their role in the CNS. We show that CSF-cNs are distributed throughout the spinal cord with a uniform morphology and a primarily ventral localization. They exhibit region-specific properties, expression of voltage-dependent and ligand-gated conductances and detect variation in extracellular pH through activation of PKD2L1 and Acid-sensing Ion Channels. They possess GABA(B) and muscarinic receptors, not glutamatergic metabotropic ones, to modulate Ca(2+) channels. CSF-cNs represent unique sensory neurons with a uniform morphology and electrophysiological properties that appear specific to the spinal cord segment inserted in. The future challenges in the field, will be to elucidate the physiological stimuli activating CSF-cNs and the neuronal network they are integrated in to modulate body function through specific local spinal network. |
