| First Author | Jørgensen HF | Year | 2010 |
| Journal | Nature | Volume | 467 |
| Issue | 7311 | Pages | E3-4; discussion E5 |
| PubMed ID | 20811409 | Mgi Jnum | J:163999 |
| Mgi Id | MGI:4830392 | Doi | 10.1038/nature09305 |
| Citation | Jorgensen HF, et al. (2010) Can controversies be put to REST?. Nature 467(7311):E3-4; discussion E5 |
| abstractText | The contribution of REST to embryonic stem (ES) cell pluripotency has been uncertain. Two years ago, Singh et al. claimed that Rest(+/-) and REST knock-down ES cells expressed reduced levels of pluripotency markers, in contrast to a prior and subsequent reports. To understand the basis of this difference, we analysed the YHC334 (YHC) and RRC160 (RRC) gene-trap ES cell lines used by Singh et al., obtained directly from BayGenomics. Both REST mutant lines generated REST-betaGeo fusion proteins, but expressed pluripotency genes at levels similar to appropriately matched parental wild ES cells, consistent with expression being REST-independent. |
