| Primary Identifier | MGI:6448946 | Allele Type | Targeted |
| Attribute String | No functional change, Reporter | Gene | Pomc |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An optimized internal ribosome entry sequence (IRES) fused to an enhanced green fluorescent protein (eGFP) sequence, followed by a FRTflanked neomycin selection cassette, was first inserted 3 bp after the Pomc stop codon within a Pomc gene. Heterozygous mice for the targeting event were crossed with mice expressing Flp-recombinase in the germline to remove neo cassette flanked by FRT sites. |
