First Author | Katada Y | Year | 2023 |
Journal | iScience | Volume | 26 |
Issue | 10 | Pages | 107716 |
PubMed ID | 37720108 | Mgi Jnum | J:340756 |
Mgi Id | MGI:7530473 | Doi | 10.1016/j.isci.2023.107716 |
Citation | Katada Y, et al. (2023) Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice. iScience 26(10):107716 |
abstractText | Photoreception requires amplification by mammalian rhodopsin through G protein activation, which requires a visual cycle. To achieve this in retinal gene therapy, we incorporated human rhodopsin cytoplasmic loops into Gloeobacter rhodopsin, thereby generating Gloeobacter and human chimeric rhodopsin (GHCR). In a murine model of inherited retinal degeneration, we induced retinal GHCR expression by intravitreal injection of a recombinant adeno-associated virus vector. Retinal explant and visual thalamus electrophysiological recordings, behavioral tests, and histological analysis showed that GHCR restored dim-environment vision and prevented the progression of retinal degeneration. Thus, GHCR may be a potent clinical tool for the treatment of retinal disorders. |